Exploration of The Natural Extender for Dilution of Walking Catfish Clarias batrachus Sperm in Refrigerated Storage

Abstract Walking catfish Clarias batrachus is a freshwaterr fish that has high economic value. Currently, the culture of this local been initiated in Indonesia, but breeding technology not well developed. Therefore, study aims to explore type natural extender for sperm preservation C. so it can support development its technology, especially remote areas. The research was conducted at Hatchery Laboratory Faculty Marine and Fisheries, Universitas Syiahh Kuala, Banda Aceh, Indonesia.. Four types extenders were tested study, namely young coconut water, nira sugarcane old water. Completely randomized design method consisting five treatments three replications used study. Sperm diluted with each dilution rate 1:20 (sperm: extender, v/v), then stored 4 °C. motility viability monitored every 24 h interval 6 days. results showed significantly affected motility, viability, fertility walking (P<0.05). Young water produced higher on all observation egg last day experiment also found values 45.83%, 43.83%, 41.50%, respectively. These different from other extenders. concluded most suitable